A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

Ünal Bakal; Mehmet Sarac; Tugay Tartar; Dilara Kaman; Ahmet Kazez

doi:10.5114/ceji.2019.89592

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Experimental immunology

A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

Ünal Bakal

¹

,

Mehmet Sarac

¹

,

Tugay Tartar

¹

,

Dilara Kaman

²

,

Ahmet Kazez

¹

Department of Pediatric Surgery, Faculty of Medicine, Firat University, Elaz|gˇ, Turkey
Department of Medical Biochemistry and Clinical Biochemistry, Faculty of Medicine, Firat University, Elazlgˇ, Turkey

(Centr Eur J Immunol 2019; 44 (3): 219-225)

DOI: https://doi.org/10.5114/ceji.2019.89592

Online publish date: 2019/09/30

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This study investigated changes in the concentrations of serum and urine neutrophil gelatinase lipocalin (NGAL), kidney injury molecule 1 (KIM-1), interleukin 18 (IL-18), and cystatin-C (Cys-C) induced by parenchymal and tubular damage following blunt kidney trauma, as well as their potential utility as biomarkers in the detection and follow-up of patients with suspected blunt renal trauma. Three-month-old male Sprague-Dawley rats (n = 18) were divided into three groups (n = 6 in each): group 1: control group (no intervention); group 2: sham group (explorative surgery and exposure of the left kidneys); and group 3: trauma group (explorative surgery and induction of blunt renal trauma of the left kidneys). Serum and urine samples were collected before and 12-24, 36-48, and 60-72 hours later for NGAL, KIM-1, IL-18, and Cys-C measurements. In the trauma group, there was a statistically significant increase in post-operative NGAL, KIM-1, and IL-18 values after 12-24 h and 36-48 h, as compared with pre-operative values. There was also a statistically significant increase in post-operative serum and urine Cys-C values after 60-72 h, as compared with pre-operative values. NGAL, KIM-1, and IL-18 may represent novel non-invasive descriptive candidate biomarkers of early-stage tubular damage in children with renal trauma.

keywords:

A study of the utility of novel non-invasive urinary and serum biomarkers of blunt kidney injury in a rat model: NGAL, KIM-1, and IL-18

Ünal Bakal 1 , Mehmet Sarac 1 , Tugay Tartar 1 , Dilara Kaman 2 , Ahmet Kazez 1

biomarkers, blunt kidney injury, neutrophil gelatinase lipocalin, kidney injury molecule 1, interleukin 18, cystatin-C

Ünal Bakal

¹

,

Mehmet Sarac

¹

,

Tugay Tartar

¹

,

Dilara Kaman

²

,

Ahmet Kazez

¹