CD163 and CCR7 as markers for macrophage polarisation in lung cancer microenvironment

Iwona Kwiecień; Małgorzata Polubiec-Kownacka; Dariusz Dziedzic; Dominika Wołosz; Piotr Rzepecki; Joanna Domagała-Kulawik

doi:10.5114/ceji.2019.92795

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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4/2019
vol. 44

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abstract:

Clinical immunology

CD163 and CCR7 as markers for macrophage polarisation in lung cancer microenvironment

Iwona Kwiecień

¹

,

Małgorzata Polubiec-Kownacka

²

,

Dariusz Dziedzic

²

,

Dominika Wołosz

³

,

Piotr Rzepecki

⁴

,

Joanna Domagała-Kulawik

⁵

Department of Internal Medicine and Hematology, Laboratory of Flow Cytometry, Military Institute of Medicine, Warsaw, Poland
Department of Surgery, Institute of Tuberculosis and Lung Diseases, Warsaw, Poland
Department of Pathology, Medical University of Warsaw, Warsaw, Poland
Department of Internal Medicine and Hematology, Military Institute of Medicine, Warsaw, Poland
Department of Internal Medicine, Pulmonary Diseases, and Allergy, Medical University of Warsaw, Warsaw, Poland

(Centr Eur J Immunol 2019; 44 (4): 395-402)

DOI: https://doi.org/10.5114/ceji.2019.92795

Online publish date: 2020/01/20

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Introduction
M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF).

Aim of the study
To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from the opposite, healthy lung, as a control, from the same patient, regarding their individual macrophage polarization and their correlation with IL-10 and TGF-.

Material and methods
Eighteen patients with confirmed lung cancer were investigated. Macrophage subtyping was performed by immunofluorescence with antibodies anti-CCR7 and CD163 (M1 and M2, respectively).

Results
We found five populations of macrophages: cells with a single reaction: only for CCR7+ or CD163+, a double reaction (CCR7+CD163+), cells with a stronger CD163 (CCR7lowCD163+), and cells with a stronger CCR7 (CCR7+CD163low). The main population in the clBALF was composed of cells with a phenotype similar to M2 (CCR7lowCD163+), while in the hlBALF the predominating phenotype was the one similar to M1 (CCR7+CD163low). The median proportion of TGF-1 concentration was higher in the clBALF and hlBALF supernatant than in the serum.

Conclusions
In this study we confirmed the usefulness of the immunofluorescence method with CCR7 and CD163 in the evaluation of BALF macrophage polarization in lung cancer.

keywords:

CD163 and CCR7 as markers for macrophage polarisation in lung cancer microenvironment

Iwona Kwiecień 1 , Małgorzata Polubiec-Kownacka 2 , Dariusz Dziedzic 2 , Dominika Wołosz 3 , Piotr Rzepecki 4 , Joanna Domagała-Kulawik 5

M1 macrophages, M2 macrophages, bronchoalveolar lavage fluid, CCR7, TGF-1, lung cancer

Iwona Kwiecień

¹

,

Małgorzata Polubiec-Kownacka

²

,

Dariusz Dziedzic

²

,

Dominika Wołosz

³

,

Piotr Rzepecki

⁴

,

Joanna Domagała-Kulawik

⁵