Experimental immunology<br>Differential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells

Joanna Chorostowska-Wynimko; Marta Kędzior; Radosław Struniawski; Beata Gruber; Elżbieta Anuszewska; Ewa Skrzypczak-Jankun; Jerzy Jankun

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ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Experimental immunology
Differential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells

Joanna Chorostowska-Wynimko

,

Marta Kędzior

,

Radosław Struniawski

,

Beata Gruber

,

Elżbieta Anuszewska

,

Ewa Skrzypczak-Jankun

,

Jerzy Jankun

(Centr Eur J Immunol 2010; 35 (3): 123-127)

Online publish date: 2010/10/05

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AMA

Chorostowska-Wynimko J, Kędzior M, Struniawski R, et al. Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells. Central European Journal of Immunology. 2010;35(3).

APA

Chorostowska-Wynimko, J., Kędzior, M., Struniawski, R., Gruber, B., Anuszewska, E.,  & Skrzypczak-Jankun, E. et al. (2010). Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells. Central European Journal of Immunology, 35(3).

Chicago

Chorostowska-Wynimko, Joanna, Marta Kędzior, Radosław Struniawski, Beata Gruber, Elżbieta Anuszewska, Ewa Skrzypczak-Jankun,  and Jerzy Jankun. 2010. "Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells". Central European Journal of Immunology 35 (3).

Harvard

Chorostowska-Wynimko, J., Kędzior, M., Struniawski, R., Gruber, B., Anuszewska, E., Skrzypczak-Jankun, E.,  and Jankun, J. (2010). Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells. Central European Journal of Immunology, 35(3).

MLA

Chorostowska-Wynimko, Joanna et al. "Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells." Central European Journal of Immunology, vol. 35, no. 3, 2010.

Vancouver

Chorostowska-Wynimko J, Kędzior M, Struniawski R, Gruber B, Anuszewska E, Skrzypczak-Jankun E et al. Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells. Central European Journal of Immunology. 2010;35(3).

The plasminogen activator inhibitor (PAI-1) is the key component of fibrynolytic system, its synthesis is rapidly activated by cytokines and mediators driving inflammatory response, both acute and persistent low-grade. While tumor necrosis factor a (TNF-α) and transforming growth factor β (TGF-b) are established potent direct stimulators of PAI-1 production in numerous tissues, little is known of any feed-back regulation that might be accountable for effects of PAI-1 on their synthesis. Present study provides evidence for the existence of negative feed-back regulation between PAI-1 and its potent activator TGF-b in endothelial cells. Dose-dependent inhibition of TGF-b production was observed in HUVEC cultures with physiological (10 mg/ml) and supraphysiological (100 mg/ml) (p < 0.02) PAI-1 concentrations exerting significant suppressive effect in comparison to control (respectively p < 0.05; p < 0.02) as well as cultures spiked with subphysiological PAI-1 dose of 1 mg/ml (p < 0.0001; p < 0.02). No such regulation was demonstrated for cancer cells of lung and prostate origin which might implicate different regulatory mechanism in neoplastic cells. Similarly, no functional interplay between PAI-1 and TNF-α levels in 24 hrs cultures of any evaluated cell lines was observed.

keywords:

Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells

Joanna Chorostowska-Wynimko , Marta Kędzior , Radosław Struniawski , Beata Gruber , Elżbieta Anuszewska , Ewa Skrzypczak-Jankun , Jerzy Jankun

PAI-1, TNF-α, TGF-b, HUVECs, lung cancer cells, prostate cancer cells

Experimental immunology
Differential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells

Joanna Chorostowska-Wynimko

,

Marta Kędzior

,

Radosław Struniawski

,

Beata Gruber

,

Elżbieta Anuszewska

,

Ewa Skrzypczak-Jankun

,

Jerzy Jankun