Experimental immunology<BR>The role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site

Yuliya I. Petrova; Olena V. Kameneva; Anastasiya I. Zhukova; Lesley Scudder; Dmitriy V. Gnatenko; Wadie F. Bahou; Sergiy V. KOMomisarenko; Maryna V. Skok

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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1/2008
vol. 33

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abstract:

Experimental immunology
The role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site

Yuliya I. Petrova

,

Olena V. Kameneva

,

Anastasiya I. Zhukova

,

Lesley Scudder

,

Dmitriy V. Gnatenko

,

Wadie F. Bahou

,

Sergiy V. KOMomisarenko

,

Maryna V. Skok

(Centr Eur J Immunol 2008; 33 (1): 14-18)

Online publish date: 2008/03/25

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AMA

Petrova Y, Kameneva O, Zhukova A, et al. Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site. Central European Journal of Immunology. 2008;33(1):14-18.

APA

Petrova, Y., Kameneva, O., Zhukova, A., Scudder, L., Gnatenko, D.,  & Bahou, W. et al. (2008). Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site. Central European Journal of Immunology, 33(1), 14-18.

Chicago

Petrova, Yuliya I., Olena V. Kameneva, Anastasiya I. Zhukova, Lesley Scudder, Dmitriy V. Gnatenko, Wadie F. Bahou,  and Sergiy V. KOMomisarenko et al. 2008. "Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site". Central European Journal of Immunology 33 (1): 14-18.

Harvard

Petrova, Y., Kameneva, O., Zhukova, A., Scudder, L., Gnatenko, D., Bahou, W., KOMomisarenko, S.,  and Skok, M. (2008). Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site. Central European Journal of Immunology, 33(1), pp.14-18.

MLA

Petrova, Yuliya I. et al. "Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site." Central European Journal of Immunology, vol. 33, no. 1, 2008, pp. 14-18.

Vancouver

Petrova Y, Kameneva O, Zhukova A, Scudder L, Gnatenko D, Bahou W et al. Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site. Central European Journal of Immunology. 2008;33(1):14-18.

Thrombin produced upon tissue damage provides a link between inflammation/blood coagulation and immune reactions through proteinase-activated receptor (PAR) types 1, 3 and 4. In contrast to PAR1 and PAR4, functions of PAR3 in cells other than platelets are poorly understood. We generated mAb 8E8 against fragment 31-47, which includes the thrombin cleavage site of human PAR3. MAb 8E8 bound mouse platelets and delayed their aggregation induced by thrombin. This antibody also specifically stained B-lymphocyte-derived hybridoma in flow cytometry. Thrombin dose-dependently inhibited hybridoma cell proliferation and so did mAb 8E8 and PAR3 31-47 fragment, but not the PAR4 activating peptide. It is concluded that mAb 8E8 recognizes PAR3 expressed in hybridoma and mimics the inhibitory effect of thrombin on hybridoma proliferation mediated by PAR3. The data obtained also suggest that, unlike in platelets, targeting PAR3 in mouse B lymphocyte-derived cells doesn’t involve PAR4.

keywords:

Experimental immunologyThe role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site

Yuliya I. Petrova , Olena V. Kameneva , Anastasiya I. Zhukova , Lesley Scudder , Dmitriy V. Gnatenko , Wadie F. Bahou , Sergiy V. KOMomisarenko , Maryna V. Skok

proteinase-activated receptor, thrombin, monoclonal antibody, hybridoma, proliferation

Experimental immunology
The role of proteinase-activated receptor 3 (PAR3) in mouse hybridoma studied with monoclonal antibody generated against thrombin cleavage site

Yuliya I. Petrova

,

Olena V. Kameneva

,

Anastasiya I. Zhukova

,

Lesley Scudder

,

Dmitriy V. Gnatenko

,

Wadie F. Bahou

,

Sergiy V. KOMomisarenko

,

Maryna V. Skok