eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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4/2020
vol. 45
 
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abstract:
Experimental immunology

Expression and regulation of macrophage-inducible C-type lectin in human synovial macrophages

Mitsutoshi Moriya
1
,
Kentaro Uchida
1, 2
,
Shotaro Takano
1
,
Dai Iwase
1
,
Gen Inoue
1
,
Manabu Muaki
1
,
Ryo Tazawa
1
,
Jun Aikawa
1
,
Hiroyuki Sekiguchi
2
,
Masashi Takaso
1

  1. Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan
  2. Shonan University of Medical Sciences Research Institute, Chigasaki City, Kanagawa, Japan
Cent Eur J Immunol 2020; 45 (4): 377-381
DOI: https://doi.org/10.5114/ceji.2020.103411
Online publish date: 2021/01/30
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Recent evidence suggests that synovial macrophage activation may be involved in cartilage destruction and pain in osteoarthritis (OA). The macrophage-inducible C-type lectin (Mincle) Clec4e is expressed in macrophages and is regulated in inflammatory conditions. Given that the regulation of Mincle in synovial macrophages has not been elucidated, we investigated the expression and regulation of Mincle in human synovial tissue (ST) harvested from patients with radiographic knee OA during total knee arthroplasty. Immunohistochemical and flow cytometric analyses were used to identify cells with Mincle expression in resected tissues. CD14-positive (CD14+; macrophage-rich cell fraction) and CD14-negative (CD14; fibroblast-rich cell fraction) cells were extracted from the ST and used to assess MINCLE mRNA expression levels. To determine the role of tumor necrosis factor alpha (TNF-) in the regulation of MINCLE expression, TNF- was used to stimulate cultured CD14+ cells. Immunohistochemical staining revealed Mincle-positive cells in the synovial lining layer. Flow cytometric analysis showed that CD45+CD14+ cells were Mincle positive while CD45–/CD14– cells were Mincle negative. MINCLE expression was significantly higher in CD14+ cells than in CD14– cells. Stimulation of cultured CD14+ macrophages with TNF- significantly increased MINCLE mRNA expression, while stimulation with TNF- neutralizing antibody significantly decreased expression. That Mincle expression was observed in synovial macrophages and its expression was induced by TNF- suggests that Mincle might have a key role in synovial inflammation in the osteoarthritic synovium.
keywords:

osteoarthritis, synovial tissue, Mincle, tumor necrosis factor-α
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