Introduction
Viral myocarditis (VMC) is the primary cause of sudden death and heart failure in adolescents [1, 2]. However, no effective treatment is available because the pathogenesis of VMC is unclear. It is widely believed that pathogenesis is chiefly due to an excessive immune response to viral infection [3], in which myocardial damage during acute VMC is due to T lymphocytes. T-lymphocyte activation requires not only T-cell receptor recognition of the major histocompatibility complex (MHC)-viral peptide complex on antigen-presenting cells (APCs), but also T-cell CD28 interaction with B7, on APCs [4].
CD80 and CD86 belong to the B7 family of costimulatory molecules and play a key role in T-cell activation and regulation [5]. Studies have shown that activated B cells, T cells, and dendritic cells express high levels of CD80 and CD86 [6-8], whereas resting B cells, T cells, and dendritic cells do not express high levels of these molecules. Seko et al. [9, 10] found that CD80 and CD86 were expressed on ventricular tissues of mice and humans with acute myocarditis as well as in humans with dilated cardiomyopathy. Although those findings indicated that CD80 and CD86 could play an important role in myocardial injury, the studies did not identify the cellular source for CD80 and CD86. Others have shown CD80 and CD86 to be expressed on disease-involved B cells in multiple sclerosis [11], rheumatoid arthritis [12], and asthma [13]. In particular, O’Neill et al. [14] demonstrated CD80+/CD86+ B cells to play a crucial role in the development of arthritis. However, little is known about the potential role of CD80+/CD86+ B cells in VMC. In this study the expression of CD80 and CD86 on B cells was investigated in a coxsackievirus B3 (CVB3)-induced acute VMC murine model, to assess possible clinical treatment modalities for VMC.
Material and methods
Mice
Specific pathogen-free, male, four-week-old C57BL/6 mice were obtained from the Laboratory Animal Centre of Foshan (Guangdong Province, China). All animals were housed in a pathogen-free mouse room in the Guangxi Medical University Laboratory Animal Centre (Nanning, China). Experiments were implemented according to protocols approved by Guangxi Medical University Animal Ethics Committee.
Virus
CVB3 (Nancy strain) was passaged in HEp-2 cells. Virus titres were assessed with plaque-forming unit (PFU) assays at 1 × 107. C57BL/6 mice were infected intraperitoneally with 105 PFU of CVB in 0.1 ml phosphate-buffered saline (PBS) to induce the acute VMC model.
Myocarditis model
A total of 36 male C57BL/6 mice were randomly divided into two groups: the VMC group (n = 20, 10 mice/group) and the control group (n = 16, 8 mice/group). Each group was divided into two subgroups: one week (1w) and two weeks (2w). Mice intraperitoneally injected with 0.1 ml of PBS containing ~1 × 105 PFU of CVB3 were the VMC group. Mice treated intraperitoneally with 0.1 ml PBS were the control group. Surviving animals of each group were euthanised at 1w and 2w after injection. Hearts as well as spleens were removed aseptically, immediately after euthanasia, for further assessment.
Histopathology
The heart was cut longitudinally, fixed in 4% paraformaldehyde, and embedded in paraffin. Tissues were cut into 5 µm-thick sections and processed by haematoxylin and eosin (H & E) staining. Histopathological changes were observed under light microscopy (magnification 400×). Myocardial pathology scores were determined by two independent investigators according to a semi-quantitative scale [15]: grade 0, no cardiac inflammation; grade 1, < 25%; grade 2, between 25% and 50%; grade 3, between 50% and 75%; and grade 4, > 75%.
Flow cytometry
Spleens were harvested aseptically and dissected from surviving mice. Cell suspensions were prepared by passage through nylon mesh, and red cells were removed with Lysing Buffer (BD). Splenocytes were resuspended as single cells in PBS and assessed with flow cytometry using the following antibodies: FITC-CD19, PE-CD86, APC-CD80, which were all purchased from eBioscience. The cells were stained with these antibodies for 30 min at 4°C in the dark. Isotype control antibodies (eBioscience) were used to control for non-specific antibody binding to the cells. Cells were then washed, resuspended in PBS, and analysed with a BD FACSCanto II flow cytometer. Data were analysed with FlowJo7.6 software.
Results
VMC severity
There were nine survivors in the 1w VMC subgroup and in the 2w VMC subgroup, respectively. No deaths were observed in the control group. For the 1w VMC subgroup there was extensive heart inflammatory cell infiltration and necrosis. By contrast, there was little less inflammatory cell infiltration and necrosis in the hearts of the 2w VMC subgroup. No inflammatory cell infiltration or necrosis was observed in the hearts of the control group (Fig. 1A). The myocardial pathology scores for the VMC groups are summarised in Figure 1B.
CD80+ B cells, not CD86+ B cells, were elevated in VMC
To quantify the surface expression of CD80 and CD86 on B cells in the acute VMC mice, the frequency of CD80+/CD86+ B cells was measured with flow cytometry (Fig. 2A, B). The frequency of CD80+ B cells was significantly increased in the VMC group compared to the control group at 1w and 2w (7.62 ±0.47% vs. 4.29 ±0.52%; 5.94 ±0.60% vs. 4.11% ±0.36%, respectively, p < 0.01, Fig. 2C). In contrast, the frequency of CD86+ B cells was significantly decreased in the VMC group when compared to the control group at 1w and 2w (1.57 ±0.44% vs. 3.85 ±0.59%; 1.45 ±0.42% vs. 3.63 ±0.45%, respectively, p < 0.01, Fig. 2D). In addition, the frequency of CD80+ B cells was higher at 1w in the VMC subgroup than in the 2w VMC subgroup (p < 0.01). There was no statistical difference in CD86+ B cells when the 1w VMC subgroup was compared to the 2w VMC subgroup (p > 0.05).
CD80+ B cell expression was related to VMC severity
To determine whether CD80+/CD86+B cells are associated with the severity of inflammation in VMC, flow cytometry results were related to heart tissue pathology scores. The frequency of CD80+ B cells was positively related to the severity of VMC heart pathology scores (p < 0.01) (Fig. 3), while the frequency of CD86+ B cells was not (data are not shown).
Discussion
In the pathogenesis of VMC, a number of studies have shown that T cells play an important role, while B cells do not. In this study, CD80+ B cells increased and CD86+ B cells decreased during VMC. CD80+ B cells were positively related to disease severity.
CD80 and CD86 are important costimulatory molecules. Although CD80 and CD86 provide synergistic stimulation for T-cell activation, CD80 promotes the differentiation of T cells into Th1 cells, while CD86 mainly induces Th2-mediated humoural immunity and immunoglobulin production. These costimulatory molecules play an important role in T-cell activation and immune upregulation during autoimmunity [16], with an imbalance of CD80 and CD86 associated with the occurrence of autoimmune diseases [17].
CD80 and CD86 play important roles in the immune system and cannot be replaced by other molecules [18, 19]. In animal models, CD80 is associated with pro-inflammatory effects, whereas CD86 is associated with anti-inflammatory effects [20]. Kuchroo et al. [21] demonstrated that treatment with anti-CD80 antibody reduced the incidence of experimental allergic encephalomyelitis (EAE), whereas administration of anti-CD86 antibody increased EAE disease severity. Furthermore, CD80 and CD86 function differently in different tissues [22, 23] and cells. Nolan et al. [24] demonstrated that during sepsis CD80 expression was upregulated, while CD86 expression was downregulated with differing expression of CD80/CD86 in different tissues.
The aforementioned studies analysed all APCs. More recently, CD80/CD86 expression on B cells was shown to drive autoimmune pathogenesis. Soraya et al. [11] demonstrated CD80 and CD86 on B cells to be elevated in all human T-cell lymphotropic virus 1 infected individuals. Sellebjerg et al. [11] demonstrated that CD80 expression on B cells was increased in the cerebrospinal fluid of multiple sclerosis patients with increased CD80+B cells, corresponding to increased disease severity. In patients with rheumatoid arthritis, Leng et al. [25] showed that CD86 expression was significantly decreased, while CD80 expression did not differ on peripheral blood B cells. With regard to VMC, CD80/CD86 expression on B cells has not been previously reported.
This study showed that CD80 expression on B cells was significantly elevated while CD86+ B cell expression was significantly decreased in acute VMC. In addition, we found that elevated CD80+B cells but not CD86+ B cells were associated with the severity of inflammation during VMC. These results suggest that CD80 is an important B-cell costimulatory molecule and that pro-inflammatory CD80+ B cells are more important than the anti-inflammatory CD86+B cells during acute VMC. These results, similarly to previous studies in other diseases, demonstrate differential B-cell expression of CD80/CD86 in acute VMC.