Immunological characterization of the American cockroach allergen Per a 9 expressed in baculovirus-infected insect cells

Hai-Wei Yang; Wei Zhang; Wei-Wei Ni; Min Jin; Shao-Heng He; Ji-Fu Wei

doi:10.5114/ceji.2019.89611

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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3/2019
vol. 44

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abstract:

Clinical immunology

Immunological characterization of the American cockroach allergen Per a 9 expressed in baculovirus-infected insect cells

Hai-Wei Yang

^{1, 2}

,

Wei Zhang

³

,

Wei-Wei Ni

¹

,

Min Jin

¹

,

Shao-Heng He

⁴

,

Ji-Fu Wei

¹

Research Division of Clinical Pharmacology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Department of Clinical Laboratory, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China

(Centr Eur J Immunol 2019; 44 (3): 322-326)

DOI: https://doi.org/10.5114/ceji.2019.89611

Online publish date: 2019/09/30

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American cockroach (CR) allergy has been recognized as important IgE-mediated type I hypersensitivity. Per a 9 is an arginine kinase, reacting with IgE in sera of all CR allergic Thai patients. Per a 9 gene was cloned and expressed in eukaryotic systems (baculovirus-infected insect cells). The expressed Per a 9 was purified by Nickel column. The antigenicities were analyzed by ELISA, immunoblot analysis and basophile activation test. The results show that 13 out of 16 (81.3%) sera from American CR patients reacted to Per a 9, confirming that Per a 9 is a major allergen of CR. The IgE reactivity of Per a 9 in the sera from American CR patients was increased 8.3-fold in comparison with the sera from healthy controls. Per a 9 at 1.0 µg/ml induced an approximately up to 5.6-fold increase in CD63 and CCR3 double positive cells when incubating with passively sensitized basophils from by sera from American CR patients.

keywords:

Immunological characterization of the American cockroach allergen Per a 9 expressed in baculovirus-infected insect cells

Hai-Wei Yang 1, 2 , Wei Zhang 3 , Wei-Wei Ni 1 , Min Jin 1 , Shao-Heng He 4 , Ji-Fu Wei 1

American cockroach, allergen, Per a 9, insect cell, IgE reactivity

Hai-Wei Yang

^{1, 2}

,

Wei Zhang

³

,

Wei-Wei Ni

¹

,

Min Jin

¹

,

Shao-Heng He

⁴

,

Ji-Fu Wei

¹