Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status

Hosni A. M. Hussein; Ali A. A. Thabet; Taha I. A. Mohamed; Mohamed E. Elnosary; Ali Sobhy; Ahmed M. El-Adly; Ahmed A. Wardany; Elsayed K. Bakhiet; Magdy M. Afifi; Usama M. Abdulraouf; Samah . M. Fathy; Noha G. Sayed; Asmaa M. Zahran

doi:10.5114/ceji.2023.129981

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Clinical immunology

Phenotypical changes of hematopoietic stem and progenitor cells in COVID-19 patients: Correlation with disease status

Hosni A. M. Hussein

¹

,

Ali A. A. Thabet

²

,

Taha I. A. Mohamed

¹

,

Mohamed E. Elnosary

³

,

Ali Sobhy

⁴

,

Ahmed M. El-Adly

¹

,

Ahmed A. Wardany

¹

,

Elsayed K. Bakhiet

¹

,

Magdy M. Afifi

¹

,

Usama M. Abdulraouf

¹

,

Samah . M. Fathy

⁵

,

Noha G. Sayed

⁶

,

Asmaa M. Zahran

⁶

Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assiut, Egypt
Department of Zoology, Faculty of Science, Al-Azhar University, Assiut, Egypt
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Department of Clinical Pathology, Faculty of Medicine, Al-Azhar University, Assiut, Egypt
Department of Zoology, Faculty of Science, Fayoum University, Fayoum, Egypt
Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Cent Eur J Immunol 2023; 48 (2): 97-110

DOI: https://doi.org/10.5114/ceji.2023.129981

Online publish date: 2023/07/17

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AMA

Hussein H, Thabet A, Mohamed T, et al. Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status. Central European Journal of Immunology. 2023;48(2):97-110. doi:10.5114/ceji.2023.129981.

APA

Hussein, H., Thabet, A., Mohamed, T., Elnosary, M., Sobhy, A.,  & El-Adly, A. et al. (2023). Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status. Central European Journal of Immunology, 48(2), 97-110. https://doi.org/10.5114/ceji.2023.129981

Chicago

Hussein, Hosni A. M., Ali A. A. Thabet, Taha I. A. Mohamed, Mohamed E. Elnosary, Ali Sobhy, Ahmed M. El-Adly,  and Ahmed A. Wardany et al. 2023. "Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status". Central European Journal of Immunology 48 (2): 97-110. doi:10.5114/ceji.2023.129981.

Harvard

Hussein, H., Thabet, A., Mohamed, T., Elnosary, M., Sobhy, A., El-Adly, A., Wardany, A., Bakhiet, E., Afifi, M., Abdulraouf, U., Fathy, S., Sayed, N.,  and Zahran, A. (2023). Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status. Central European Journal of Immunology, 48(2), pp.97-110. https://doi.org/10.5114/ceji.2023.129981

MLA

Hussein, Hosni A. M. et al. "Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status." Central European Journal of Immunology, vol. 48, no. 2, 2023, pp. 97-110. doi:10.5114/ceji.2023.129981.

Vancouver

Hussein H, Thabet A, Mohamed T, Elnosary M, Sobhy A, El-Adly A et al. Phenotypical changes of hematopoietic stem
and progenitor cells in COVID-19 patients:
Correlation with disease status. Central European Journal of Immunology. 2023;48(2):97-110. doi:10.5114/ceji.2023.129981.

PlumX metrics:

Hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) play a crucial role in the context of viral infections and their associated diseases. The link between HSCs and HPCs and disease status in COVID-19 patients is largely unknown. This study aimed to monitor the kinetics and contributions of HSCs and HPCs in severe and non-severe COVID-19 patients and to evaluate their diagnostic performance in differentiating between healthy and COVID-19 patients as well as severe and non-severe cases. Peripheral blood (PB) samples were collected from 48 COVID-19 patients, 16 recovered, and 27 healthy controls and subjected to deep flow cytometric analysis to determine HSCs and progenitor cells. Their diagnostic value and correlation with C-reactive protein (CRP), D-dimer, and ferritin levels were determined. The percentages of HSCs and common myeloid progenitors (CMPs) declined significantly, while the percentage of multipotent progenitors (MPPs) increased significantly in COVID-19 patients. There were no significant differences in the percentages of megakaryocyte-erythroid progenitors (MEPs) and granulocyte-macrophage progenitors (GMPs) between all groups. Severe COVID-19 patients had a significantly low percentage of HSCs, CMPs, and GMPs compared to non-severe cases. Contrarily, the levels of CRP, D-dimer, and ferritin increased significantly in severe COVID-19 patients. MPPs and CMPs showed excellent diagnostic performance in distinguishing COVID-19 patients from healthy controls and severe from non-severe COVID-19 patients, respectively. Collectively, our study indicated that hematopoietic stem and progenitor cells are significantly altered by COVID-19 and could be used as therapeutic targets and diagnostic biomarkers for severe COVID-19.

keywords:

Phenotypical changes of hematopoietic stem and progenitor cells in COVID-19 patients: Correlation with disease status

Hosni A. M. Hussein 1 , Ali A. A. Thabet 2 , Taha I. A. Mohamed 1 , Mohamed E. Elnosary 3 , Ali Sobhy 4 , Ahmed M. El-Adly 1 , Ahmed A. Wardany 1 , Elsayed K. Bakhiet 1 , Magdy M. Afifi 1 , Usama M. Abdulraouf 1 , Samah . M. Fathy 5 , Noha G. Sayed 6 , Asmaa M. Zahran 6

COVID-19, SARS-CoV-2, hematopoietic stem cells (HSCs), hematopoietic progenitor cells (HPCs)