eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
3/2019
vol. 44
 
Share:
Share:
abstract:
Clinical immunology

Tumour necrosis factor gene polymorphisms in Egyptian patients with rheumatoid arthritis and their relation to disease activity and severity

Heba M. Zaghlol
1
,
Sanaa Abdelshafy
1
,
Rabab A. Mohamed
1
,
Enas A. Abdelaleem
2

  1. Department of Clinical Pathology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt
  2. Department of Rheumatology and Rehabilitation, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt
(Centr Eur J Immunol 2018; 44 (3): 277-284)
Online publish date: 2019/09/30
View full text Get citation
 
PlumX metrics:
Aim of the study
The present case control study was conducted to assess the association of LTA 252 A>G, TNF-α 308 G>A, and TNF-α 1031 T>C gene polymorphisms with rheumatoid arthritis (RA), and their involvement in disease activity and severity.

Material and methods
A total of 70 Egyptians, including 35 RA patients and 35 healthy control individuals, were included in the study. The RA patients comprised 34 females and one male. Cases with RA were diagnosed by a rheumatologist and fulfilled the 2010 ACR/EULAR criteria. Modified disease activity score (DAS28) was used to assess disease activity. Van Der Heijde-modified Sharp score (vdHSS) was used to assess radiological changes for assessment of disease severity. PCR-RFLP was used to detect the association of LTA 252 A>G, TNF-α 308 G>A, and TNF-α 1031 T>C gene polymorphisms with RA.

Results
TNF-α 308 G allele and TNF-α 308 GG genotype were significantly higher in RA patients compared to healthy control subjects (p = 0.04 and p = 0.001, respectively). TNF-α 308 G allele and GG genotype were significantly higher in the RA non-remission group compared to the remission group (p = 0.008, p < 0.001). Patients with the TNF-α 308 AG genotype had higher mean of Sharp score compared to the patients with the GG and AA genotypes (p = 0.007). There was no significant association between LTA 252 A>G and TNF-α 1031 T>C gene polymorphisms and RA.

Conclusions
Our results suggest that TNF-α 308 G/A gene polymorphism is genetically associated with RA and involved in disease activity and severity in Egyptian patients.

keywords:

rheumatoid arthritis, genetic susceptibility, TNF polymorphisms, polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP)

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.